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Connecting nuclear architecture and genomic function

✍ Scribed by Ronald Berezney; Michael Mortillaro; Hong Ma; Chunling Meng; Jagath Samarabandu; Xiangyun Wei; Suryanarayan Somanathan; W.S. Liou; S.J. Pan; Ping-chin Cheng

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 351 KB
Volume: 62
Category: Article
ISSN: 0730-2312
DOI: 10.1002/(sici)1097-4644(199608)62:2<223::aid-jcb10>3.0.co;2-m

No coin nor oath required. For personal study only.

✦ Synopsis

Research in our laboratory employs a dual approach to correlating genomic function and regulation with nuclear architecture. In one series of projects we are identifying, cloning, and studying the molecular, genetic, and functional properties of the nuclear matrix proteins which comprise the three-dimensional nuclear architecture. In a second series, we are using molecular labeling, microscopic, and computer imaging approaches to visualize in three dimensions sites of genomic organization and function within the in situ nuclear architecture and to obtain precise structural and spatio-temporal information about the individual sites where genomic function and regulation occurs. Additionally, our group is investigating the possible arrangement of the functional sites into higher-order domains and their relationship to the overall architecture and regulation in the cell nucleus.

Nuclear Matrix Proteins as a Basis for Cenomic Organization, Function, and Regulation

While a plethora of functional properties are associated with the isolated nuclear matrix [Berezney, 1991; Berezney et al., 19951, our knowledge of the proteins which compose the nuclear matrix is still in its infancy. Comprehensive analysis of the individual proteins is, therefore, critical as a basis for correlating higher order structural organization in the nucleus with the associated genomic functions.

Studies in our laboratory are emphasizing the major proteins of the nuclear matrix (termed nuclear matrins) which are common among mammalian cells [Nakayasu and Berezney, 1991; Belgrader et al., 1991al. Microsequencing, molecular cloning and immunoblot analysis has

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