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The need for direct cell contact in “contact” inhibition of cell division in culture

✍ Scribed by Leonard Schutz; Peter T. Mora

Publisher
John Wiley and Sons
Year
1968
Tongue
English
Weight
460 KB
Volume
71
Category
Article
ISSN
0021-9541

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✦ Synopsis

Non-dividing mouse embryo fibroblasts which grew to a confluent cell density on one side of an ultra-thin filter did not inhibit the active multiplication of the same type of cells growing at low cell density on the other side of the filter directly opposite the confluent side. The close proximity of the cells across the filter was not sufficient to cause inhibition of cell division. The phenomenon of "contact" or "density dependent" inhibition of cell division is therefore probably not mediated by a cellular product which remains concentrated near the cell surface.

The degree of contact inhibition of cell division was correlated with the local cell density on the same side of the filter. This relationship was found to be influenced strongly by the surface on which the cells were growing.

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