Direct cell contact is required in the syngeneic mixed lymphocyte reaction

✍ Scribed by H. Von Boehmer

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: English
• Weight: 320 KB
• Volume: 3
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830030211

✦ Synopsis

Abstract

A positive syngeneic mixed lymphocyte reaction can be observed by culturing neonatal thymus cells with syngeneic Mitomycin C‐treated spleen cells. Evidence that direct cell contact between the reacting thymus cells and the stimulating spleen cells is required was obtained by the following experiments. Supernatants from spleen cells prepared after different times of culture failed completely to stimulate syngeneic thymus cells. Stimulation was not transmitted in a double chamber system where thymus cells and spleen cells were separated by a nucleopore membrane (pore size 0.22 μ).

Fetal calf serum was not required in the culture medium to obtain a positive syngeneic and allogeneic mixed lymphocyte reaction, indicating that foreign protein does not influence the reaction.