## Abstract The intracellular compartmentation of pyruvate in primary cultures of cortical neurons was investigated by high resolution ^13^C NMR using mixtures of different pyruvate precursors conveniently labeled with ^13^C or unlabeled. Cells were incubated with 1–5 mM (1‐^13^C, 1,2‐^13^C~2~ or U
The nature of pyruvate bound to pyruvate kinase as determined by 13C NMR spectroscopy
✍ Scribed by Jonathan J. Burbaum; Jeremy R. Knowles
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 796 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0045-2068
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📜 SIMILAR VOLUMES
## Abstract The assignment of backbone resonances and the secondary structure determination of the Cys 10 Ser mutant of enzyme IIB^cellobiose^ of the __Escherichia coli__ cellobiose‐specific phosphoenol‐pyruvate‐dependent phosphotransferase system are presented. The backbone resonances were assigne
13C NMR spectroscopy can be used for the quantitative analysis of mixtures of glycerides and the enantiomeric purity can be determined by using Eu(hfbc),-d reagent. This technique was applied to the study of the reaction mixture obtained from the transesterification of tributyrin catalysed by My-lip