The N-terminal region of eukaryotic translation initiation factor 5A signals to nuclear localization of the protein
β Scribed by Lucas T. Parreiras-e-Silva; Marcelo D. Gomes; Eduardo B. Oliveira; Claudio M. Costa-Neto
- Book ID
- 116295475
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 586 KB
- Volume
- 362
- Category
- Article
- ISSN
- 0006-291X
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Eukaryotic initiation factor 5A (eIF-5A) is the only protein in nature that contains hypusine, an unusual amino acid residue formed posttranslationally by deoxyhypusine synthase and deoxyhypusine hydroxylase. Although the eIF-5A gene is essential for cell survival and proliferation, the precise func
## Abstract A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from __Methanococcus jannaschii__, a hyperthermophile with an optimum growth temperature of 85 Β°C. The protein was overexpressed, purified and crystallized. The crystals were obtai