Resonance assignment for the N-terminal region of the eukaryotic initiation factor 5 (eIF5)

Cloning, expression, and crystallization

Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A

✍ Kyeong Kyu Kim; Hisao Yokota; Rosalind Kim; Sung-Hou Kim 📂 Article 📅 2008 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 266 KB

## Abstract A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from __Methanococcus jannaschii__, a hyperthermophile with an optimum growth temperature of 85 °C. The protein was overexpressed, purified and crystallized. The crystals were obtai