The mitochondrially encoded “phantom ATPase” of S. cerevisiae
✍ Scribed by Somlo, Marie ;Krupa, Mireille
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 592 KB
- Volume
- 167
- Category
- Article
- ISSN
- 0026-8925
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📜 SIMILAR VOLUMES
The ENA2 gene encoding a P-type ATPase involved in Na+ and Li+ effluxes in Saccharomyces cerevisiae has been isolated. The putative protein encoded by ENA2 differs only in thirteen amino acids from the protein encoded by ENA1/PMR2. However, ENA2 has a very low level of expression and for this reason
1. The distribution of ATPase and several marker enzymes was examined after differential and sucrose gradient centrifugation of yeast homogenates. 2. An ATPase activity not sensitive to oligomycin is found exclusively associated with a particulate fraction equilibrating at densities of 1.23-1.25. Th