An Aspergillus nidulans gene library was constructed in a high-frequency transformation vector, pDJB3, based on the Neurospora crassa pyr4 gene. This gene library was used to isolate the structural gene for isocitrate lyase (acuD) by complementation of a deficiency mutation following transformation
The metG gene of Aspergillus nidulans encoding cystathionine b-lyase: cloning and analysis
✍ Scribed by M. Sieńko; Andrzej Paszewski
- Publisher
- Springer-Verlag
- Year
- 1999
- Tongue
- English
- Weight
- 306 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0172-8083
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A cDNA clone, CGS1, encoding cystathionine "7-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGSI can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revea