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The metabolism of pyrimidine compounds by Tetrahymena pyriformis

✍ Scribed by J. R. Wykes; D. M. Prescott

Publisher: John Wiley and Sons
Year: 1968
Tongue: English
Weight: 845 KB
Volume: 72
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040720305

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✦ Synopsis

The pyrimidine requirements for growth of T. pyrifomzis and for reversal of the growth inhibition caused by folate deprivation have been studied. The effects of thymidine and 5-fluorodeoxyuridine have been shown to be quantitatively different from the effects of these compounds on growth and the rate of DNA synthesis in mammalian cells. Labelled nucleosides added to the medium have been found to be converted to the corresponding bases with the exception of deoxycytidine, which is first deaminated to deoxyuridine. As a result no deoxynucleosides other than thymidine specifically label DNA.

The results allow deductions to be made concerning the enzymes involved in pyrimidine utilization by this organism. It is suggested that pyrimidine utilization is always channeled through uracil in the case of those compounds that can supply the pyrimidine requirement for growth.

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