Some aspects of lipid metabolism in Tetrahymena pyriformis GL during enviromental changes

✍ Scribed by E. G. Koch; O. H. Scherbaum

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 863 KB
• Volume: 7
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19670070504

✦ Synopsis

Lipid content and fatty acid composition of Tetrahymena can be affected by both the incubation temperature and the composition of the growth medium. The lipid content of 17.5% in proteose-peptone grown cells increases to 44.8% by the addition of Tween 80 to the culture medium (KIDDER et al., 1954). The use of different carbon sources such as acetate, propionate, iso-butyrate and a-methyl-n-butyrate in synthetic media results in alterations in the fatty acid composition of Tetrahymena lipids (SIIORB, 1963). Cultures grown a t 35" contain more lauric, myristic and palmitic acids than cultures grown a t 25' (ERWIN and BLOCH, 1984).

Cytological studies of synchronously dividing Tetrahymena revealed a decrease in lipid content as the cells recover from the heat treatment (ENGEMANN, 1958). A high lipid

I. Material and methods

Cell g r o w t h a n d h a r v e s t p r o c e d u r e . The ciliate protozoan Tetrahyniena 1iyt-iforntis BL was grown in a 2% (w/v) proteose-peptone medium (DIFCO Laboratories) with 0.176 liver extract S (WILSON Laboratories), 0.5% bacto-dextrose (DIFCO Laboratories),