The metabolism of norethandrolone in the horse: characterization of 16-, 20- and 21-oxygenated metabolites by gas chromatography/mass spectrometry

✍ Scribed by Andrew R. McKinney; Damon D. Ridley; Craig J. Suann

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 148 KB
• Volume: 36
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.111

✦ Synopsis

Abstract

After oral administration to a thoroughbred gelding, the anabolic steroid norethandrolone was converted into a complex mixture of oxygenated metabolites. These metabolites were extracted from the urine, deconjugated by methanolysis and converted to their O‐methyloxime trimethylsilyl derivatives. Gas chromatographic/mass spectrometric analysis indicated the major metabolites to be 19‐norpregnane‐3,16,17‐triols, 19‐norpregnane‐3,17,20‐triols and 3,17‐dihydroxy‐19‐norpregnan‐21‐oic acids. Some minor metabolites were also detected. Copyright © 2001 John Wiley & Sons, Ltd.