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The long-term effect of glucagon on pyruvate kinase activity in primary cultures of hepatocytes

โœ Scribed by Claudia Evans; Osamu Miyanaga; G.Larry Cottam


Book ID
115706266
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
671 KB
Volume
233
Category
Article
ISSN
0003-9861

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Long-term culture of hepatocytes: Effect
โœ John Dich; Constance Vind; Niels Grunnet ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 910 KB

Hepatocytes isolated from adult rats were cultured for 2 to 3 weeks on collagen in a modified, serum-free Waymouth medium containing fatty acids and varying concentrations of glucocorticoid, insulin and glucagon. (ii) In the presence of all three hormones, it was possible to maintain the content of