The lipopolysaccharide of the green sulfur bacteriumChlorobium vibrioformef.thiosulfatophilum

✍ Scribed by J. Meißner; U. Fischer; J. Weckesser

• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 798 KB
• Volume: 149
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00425077

✦ Synopsis

The cell wall lipopolysaccharide of the green sulfur bacterium Chlorobium vibrioforme f. thiosuIfatophilum was obtained by the phenol-chloroform-petroleum ether and the hot phenol-water methods, respectively. It contained mannose, glucose, galacturonic acid, glucosamine, glycine, and small amounts of rhamnose, galactose and glucuronic acid. In addition to D-glycero-o-mannoheptose, the corespecific constituents 2-keto-3-deoxyoctonate and L-glyceroo-mannoheptose were found. Polyacrylamide gel-electrophoresis in the presence of sodium deoxycholate gave no indication for the presence of O-specific repeating units. Degradation of the lipopolysaccharide required 10% acetic acid (100~ 2 h). The lipid A moiety contained the total of glucosamine of the lipopolysaccharide as well as small amounts of 2,3-diamino-2,3-dideoxy-glucose. It was phosphate-free. The fatty acid spectrum comprised 3-OH-14:0, 3-OH-16: 0, and iso-3 -OH-18 : 0 besides little 12 : 0, 14: 0 and 16:0. Hydroxylaminolysis and sodium methylate treatment revealed all of the three hydroxy fatty acids to be amidebound.