ATP-linked citrate lyase activity in the green sulfur bacteriumChlorobium limicolaformathiosulfatophilum
✍ Scribed by R. N. Ivanovsky; N. V. Sintsov; E. N. Kondratieva
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 259 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Cell-free extracts of the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum strains 1 C and L have been shown to cleave citrate with the formation of oxaloacetate and acetyl-CoA. This capacity was found in autotrophically grown cells as well as in the cells grown on media with acetate or Lglutamate. Citrate lyase activity in cell-free extracts is only measurable in the presence of citrate, adenosine-5'-triphosphate, coenzyme A and Mg 2 + or Mn 2 +. It is concluded on the basis of the obtained data that C. limicola f. thiosulJatophilum contains adenosine-Ytriphosphate-linked citrate lyase (E.C.4.1.3.8). In contrast to green bacteria in the purple bacteria Ectothiorhodospira shaposhnikovii, Rhodospirillum rubrum and Thiocapsa roseopersicina citrate lyase activity was not found.