The large-scale production of protein from leaf extracts
β Scribed by J. E. Morrison; N. W. Pirie
- Publisher
- John Wiley and Sons
- Year
- 1961
- Tongue
- English
- Weight
- 457 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0022-5142
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β¦ Synopsis
Abstract
Juice from fresh, pulped, leaves is freed from most of the starch grains, fibre and detritus and coagulated quickly with steam. The protein coagulum is filtered off, washed with water at pH 4 and pressed into blocks with 30β40% dry matter. In this form it keeps under refrigeration.
Texture, colour and nutritive value of the final product are determined by the conditions of drying. The precautions needed during drying, especially freezeβdrying, are described. The products are fairly stable at room temperature but lipids have to be removed before the protein will keep permanently. The conditions for doing this by acetone extraction are described.
The composition of the protein and the uses to which it can be put are described briefly.
π SIMILAR VOLUMES
transformation frequencies. About 1.5-to 3-fold higher CFU was observed with the use of 30 mM sodium citratebuffered transformation mix at pH 5.5 compared with that at neutral pH. The standard transformation protocol described here and performed with 10-fold concentrated cells was applicable to a r
Three spectrophotometric methods for the quantitative determination of proteins were compared taking as a reference the method of Kjeldahl. The abnormally high protein values obtained when these methods were applied to soluble extracts were decreased by using protective and precipitating agents. The