Large-Scale Production of Procollagen I from Chick Embryo Tendon Fibroblasts
✍ Scribed by Raquel Martin; Jean Farjanel; Denise Eichenberger; Marie-Madeleine Giraud-Guille; David J.S. Hulmes
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 65 KB
- Volume
- 277
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
transformation frequencies. About 1.5-to 3-fold higher CFU was observed with the use of 30 mM sodium citratebuffered transformation mix at pH 5.5 compared with that at neutral pH.
The standard transformation protocol described here and performed with 10-fold concentrated cells was applicable to a range of strains of E. coli, albeit with variable frequencies (Fig. ). Highest transformation frequencies (Ͼ5 ϫ 10 9 ) CFU were obtained with E. coli RR1 and with many of the other commonly used strains in our laboratory such as TB1 and XL1 frequencies ranged around 10 8 . However, some strains such as CJ236 and MRE600 proved difficult to transform possibly due to greater sensitivity of the bacteria to high concentration of glycine during culturing.
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