On binding of gadolinium ions to the terminal carboxyl group of a peptide, the spin-lattice relaxation times of the WC protons are reduced sequentially from the C terminus, thus allowing the sequence determination of submilligram amounts of peptides.
The lanthanide-induced-shift-assisted determination of proton spin-lattice relaxation times
β Scribed by Milton D Johnston Jr.; G Herbert Caines; Andrew S Zektzer
- Publisher
- Elsevier Science
- Year
- 1984
- Weight
- 700 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0022-2364
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract __T__~1~ and __T__~2~ can be rapidly determined with a combination of multiangle spoiled gradient recalled echo (SPGR) and steadyβstate free precession (SSFP) imaging. Previously, we demonstrated a simple method for determining the set of SPGR and SSFP angles that provided greater __T__
1H NMR spin-lattice relaxation times (T1) of the N-CH3 proton resonances of phosphocreatine (PCr) and creatine (Cr) in water solutions were obtained using the 1,3,3,1 pulse sequence. These T1 values were equivalent to those obtained in D2O and water using either the conventional inversion-recovery e
The activation enthalpy and entropy for ligand exchange in the weak complex Ni(glycerol)i+ were estimated by variable-temperature proton spin-lattice relaxation time measurements at 9.4 and 6.3 T on a 0.044 molal solution of NiCI, in glyceroL A description of the dynamic process in terms of a simple