✦ LIBER ✦

The isolation and nucleotide sequence of the pyruvate decarboxylase gene fromKluyveromyces marxianus

✍ Scribed by Paul Holloway; Ronald E. Subden

Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 383 KB
Volume: 24
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00351804

No coin nor oath required. For personal study only.

✦ Synopsis

We have determined the nucleotide sequence of a 2360-basepair (bp) region of the Kluyveromyces marxianus genome containing the structural gene for the enzyme pyruvate decarboxylase (PDC). Comparison of the deduced amino-acid sequence of this gene to that of the Saccharomyces eerevisiae PDC genes reveals extensive homology including a motif common to thiamin diphosphate-dependent enzymes.

📜 SIMILAR VOLUMES

Isolation and nucleotide sequence of the

Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase fromKluyveromyces lactis

✍ Kitamoto, Hiroko K.; Ohmomo, Sadahiro; Iimura, Yuzuru 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 148 KB 👁 2 views

The KlPCK1 gene encoding phosphoenolpyruvate carboxykinase (PEPCK; ATP-dependent) was cloned from the Kluyveromyces lactis genome using a PCR amplicon from Saccharomyces cerevisiae PCK1 gene as a probe. A DNA fragment of about 4•8 kb containing KlPCK1 complemented PEPCK activity of the mutant of S.

Isolation and nucleotide sequence of the

Isolation and nucleotide sequence of the 5-aminolevulinate synthase gene fromAspergillus nidulans

✍ Rosemary E. Bradshaw; Simon W. C. Dixon; Desmond C. Raitt; Timothy M. Pillar 📂 Article 📅 1993 🏛 Springer-Verlag 🌐 English ⚖ 1010 KB

The structural gene for 5-aminolevulinate (ALA) synthase has been cloned and sequenced from the filamentous fungus Aspergillus nidulans using an oligonucleotide probe based on a highly conserved-amino-acid sequence found in ALA synthase genes of a wide range of species. The cloned gene, hemA, has a

Isolation and nucleotide sequence of the

Isolation and nucleotide sequence of the potato mitochondrial gene for apocytochrome b

✍ Silvana Zanlungo; Simon Litvak; Xavier Jordana 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 248 KB

Isolation and nucleotide sequence of the

Isolation and nucleotide sequence of the hemA gene of Escherichia coli K12

✍ Drolet, Marc ;Péloquin, Luc ;Echelard, Yann ;Cousineau, Linda ;Sasarman, A. 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 614 KB

The hemA gene of Escherichia coli K12 was cloned by complementation of a hemA mutant of this organism. Subcloning of the initial 6.0 kb HindIII fragment allowed the isolation of a 1.5 kb NheI-AvaI fragment which retained the ability to complement the hemA mutant. DNA sequencing by the dideoxy chain

Isolation and sequence analysis of the o

Isolation and sequence analysis of the orotidine-5'-phosphate decarboxylase gene (URA3) ofCandida utilis. Comparison with the OMP decarboxylase gene family

✍ Rodríguez, Luis; Chávez, Francisco P.; González, Maria E.; Basabe, Liliana; Rive 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 265 KB 👁 2 views

The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the fam

Nucleotide sequence of the tryptophan de

Nucleotide sequence of the tryptophan decarboxylase gene of Catharanthus roseus and expression of tdc-gusA gene fusions in Nicotiana tabacum

✍ Goddijn, Oscar J. M. ;Lohman, Frans P. ;de Kam, Rolf J. ;hilperoort, Rob A. ;Hog 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 982 KB