A procedure is described for the isolation and purification of the DNA of plasmids that are indigenous to the agriculturally important nitrogen-fixing bacterium Rhizobium meliloti.
The isolation, analysis and chemical reactions of deoxyribonucleic acid (DNA)—I.: The isolation and purification of DNA
✍ Scribed by J.R. Tittensor; R.T. Walker
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 952 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0014-3057
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✦ Synopsis
The methods available for the isolation of DNA from various sources and in amounts varying from I kg to single intact molecules are reviewed. Emphasis has been placed on those methods which have been reported since 1960. The methods available for the separation of DNA from ribonucleic acids, polysaeeharides, proteins and other cellular material are discussed, together with some of the methods which have been proposed for separating native and denatured DNA.
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