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The investigation of single bacteria by means of fluorescence staining and Raman spectroscopy

✍ Scribed by M. Krause; B. Radt; P. Rösch; J. Popp

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
331 KB
Volume
38
Category
Article
ISSN
0377-0486

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✦ Synopsis

Abstract

The successful combination of Raman spectroscopy and fluorescence labelling of single bacterial cells is presented. The results show that the selected dyes (DAPI, SYTO 9, CFDA, SYTOX blue and PI) in most cases do not influence the Raman spectrum of a labelled bacterium significantly compared to an unstained bacterium spectrum. The labelling of bacteria offers the possibility of live/dead or biotic/abiotic differentiation prior to a Raman identification step. Copyright © 2007 John Wiley & Sons, Ltd.

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