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Investigation of Nanoparticle Coating by Fluorescence Correlation Spectroscopy

✍ Scribed by Piotr Jurkiewicz; Čestmír Koňák; Vladimír Šubr; Martin Hof; Petr Štĕpánek; Karel Ulbrich

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
243 KB
Volume
209
Category
Article
ISSN
1022-1352

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✦ Synopsis

Abstract

Protecting gene delivery vectors by hydrophilic copolymer coating is a promising approach in targeted gene therapy. Herein we demonstrate the usefulness of FCS in characterizing the coating process of a virus model – latex beads with coumarin‐labeled N‐(2‐hydroxypropyl)methacrylamide copolymer containing thiazolidine‐2‐thione groups. FCS gives an estimation of the average number of polymer chains coating the latex bead, which agrees with light scattering results, giving an evidence of quality of the vector surface modification. FCS is advantageous because it decreases the sample consumption by a factor of 1 000. The coumarin label showed advantages over the previously used fluorescein‐based one.

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