Applications of Fluorescence Correlation Spectroscopy— Particle Size Effect

✍ Scribed by Konstantin Starchev; Jingwu Zhang; Jacques Buffle

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 186 KB
• Volume: 203
• Category: Article
• ISSN: 0021-9797
• DOI: 10.1006/jcis.1998.5470

✦ Synopsis

gation of diffusion dynamics and molecular interactions of Both computer simulations and experiments have been emmacromolecules and biopolymers in solutions and on memployed to study the fluorescence correlation spectroscopy autocorbranes (7, 8). The method measures the fluorescent light relation function of particles with size comparable to the size of emitted from individual particles or molecules during their the light beam. When such particles are labeled by several fluorodiffusion through a very small (typically less than 1 mm 3 ) phores the characteristic time of diffusion and the particle number illuminated volume named the sampling volume (SV). The in the light beam are larger than those expected from the existing diffusion coefficients and the number of fluorescent moletheory developed for the case of a point size diffuser. The resulting cules in the SV are obtained from the autocorrelation funceffect is described by simple equations.