Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference
The Inherent Quantitative Capacity of the Reverse Transcription-Polymerase Chain Reaction
β Scribed by William P. Halford; Viviana C. Falco; Bryan M. Gebhardt; Daniel J.J. Carr
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 363 KB
- Volume
- 266
- Category
- Article
- ISSN
- 0003-2697
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