The influence of temperature on the water exchange in amphibian eggs and embryos

✍ Scribed by Birgit Haglund; Søren Løvtrup

• Publisher: John Wiley and Sons
• Year: 1966
• Tongue: English
• Weight: 373 KB
• Volume: 67
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040670216

✦ Synopsis

The water exchange has been measured in oocytes of Siredon mexicanum and Rana temporaria, and in unfertilized eggs and early gastrulae of the former species, by recording the D20-H20 exchange with the Cartesian diver balance.

In oocytes, where no diffusion barrier to water is demonstrable, the temperature coefficient QIO for the exchange of water is about 1.3-1.4, corresponding to that of free diffusion. In unfertilized eggs, and in early gastrulae the exchange is considerably slowed down, indicating that a surface membrane to some extent limits the rate of exchange. At the same time the QIO value is increased, lying in the range 2.3-3.8. Since it is most likely that the exchange even in this case occurs by diffusion, but through membrane pores, it is concluded that the area available for diffusion (pore size or number, or both) increases with temperature.