The influence of rotor speed on the sedimentation behavior in sucrose gradients of high molecular weight DNA's
β Scribed by Irwin Rubenstein; Sara B. Leighton
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 554 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0301-4622
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π SIMILAR VOLUMES
## Abstract Large molecular weight bacteriophage G DNA, about five times larger than T2 DNA, was used to test Zimm's theory [(1974) __Biophys. Chem.__ **1**, 279β291] for the effect of rotor speed on the sedimentation of large linear monodisperse DNA. Sedimentation profiles from neutral sucrose gra
The sedimentation properties of a P. mimbilis DNA sample have been investigated at different concentrations and rotor speeds. Pronounced speed effects occurred at high angular velocities. The s&w value evaluated from low-speed experiments amounts to 61 S., indicating a mean molecular weight of 105 m
## Abstract The problem of inferring molecular weight distributions from concentration gradient data taken at sedimentationβdiffusion equilibrium for a single rotor speed is ill posed: significantly different distributions correspond to concentration gradients which differ so slightly that they can