## Abstract Large molecular weight bacteriophage G DNA, about five times larger than T2 DNA, was used to test Zimm's theory [(1974) __Biophys. Chem.__ **1**, 279β291] for the effect of rotor speed on the sedimentation of large linear monodisperse DNA. Sedimentation profiles from neutral sucrose gra
Dependence of the sedimentation of high molecular weight DNA on centrifuge speed
β Scribed by Franklin Hutchinson; Frank Krasin
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 708 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0301-4622
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The sedimentation properties of a P. mimbilis DNA sample have been investigated at different concentrations and rotor speeds. Pronounced speed effects occurred at high angular velocities. The s&w value evaluated from low-speed experiments amounts to 61 S., indicating a mean molecular weight of 105 m
## Abstract The problem of inferring molecular weight distributions from concentration gradient data taken at sedimentationβdiffusion equilibrium for a single rotor speed is ill posed: significantly different distributions correspond to concentration gradients which differ so slightly that they can
A procedure to isolate high-molecular-weight DNA from plant materials has been devised. With this procedure, high-molecular-weight DNA suitable for Southern transfer experiments has been isolated from over 30 plant species including angiosperms (both dicots and monocots), a gymnosperm, members of ot