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The influence of initial sucrose and nitrate concentrations on the growth ofCinchona ledgerianacell suspension cultures and the production of alkloids and anthraquinones

✍ Scribed by Rommert Wijnsma; Robert Verpoorte; Peter A. A. Harkes; Theo B. Vliet; Hens J. G. Hoopen; Anders Baerheim Svendsen

Publisher: Springer
Year: 1986
Tongue: English
Weight: 453 KB
Volume: 7
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00043917

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✦ Synopsis

The influence of initial concentrations of two of the major medium components, sucrose and nitrate, on the growth and the production of alkaloids and anthraquinones in cell suspension cultures of Onchona ledgeriana Moens was studied. It was found that maximum growth and maximum alkaloid yield were obtained with a B smedium containing the normal level of nitrate and 4% sucrose, whereas the anthraquinone yield was maximum at 8% sucrose at the normal level of nitrate. Maximum contents of secondary metabolites, expressed as tag. g DW -t , were found using a medium containing 2% sucrose, but four times the normal nitrate concentration.

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