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The influence of aging on the phorbol and K+-depolarization induced phosphorylation of the protein B-50 (GAP 43)

โœ Scribed by Gianotti, C.; Bruni, A.; Toffano, G.


Book ID
122360565
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
73 KB
Volume
22
Category
Article
ISSN
1043-6618

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A combined assay to measure neurite outgrowth and B-50/GAP-43 levels in PC12 cells is reported. During NGF-induced neuritogenesis, B-50/GAP-43 expression was monitored by enzyme-linked immunosorbent assay (ELISA). Neurite outgrowth was quantified at the same time by the use of video image analysis.