The induction of a chromosomal aberration by bromouracil incorporation in Escherichia coli K 12
β Scribed by Franklin R. Leach; Dionne D. Wilson; Nancy S. Erwin; Thomas S. Matney
- Book ID
- 118848444
- Publisher
- Elsevier Science
- Year
- 1963
- Tongue
- English
- Weight
- 218 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0006-291X
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Several mutants unable to utilize trehalose were isolated from Escherichia coli, Their genetic analysis led to determine the following gene order on the chromosomal map: pur B-dad R-tre-hem a-trp. Furthermore, the tre gene belongs to the inversion of the trp chromosomal region between E. coli and S.
Various Escherichia coli K12 Hfr donors transfer at low frequency portions of the E. coli genome to Proteus mirabilis. By remating such Proteus hybrids with the same or a different E. coli Hfr strain, other genetic characters could be added to yield diploid Proteus hybrids which contained more than