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The incorporation of uracil into animal cell DNA in vitro

✍ Scribed by Grafstrom, Robert H.; Tseng, B.Y.; Goulian, Mehran

Book ID
122415197
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
756 KB
Volume
15
Category
Article
ISSN
0092-8674

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✍ FranΓ§oise Doppler-Bernardi; Gary Felsenfeld πŸ“‚ Article πŸ“… 1969 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 540 KB

An exchange method is described for producing tritium-labeled native DNA in vitro with minimal physical damage to the DNA. Tritium-labeled calf thymus DNA prepared in this way has a specific activity of about 100 pCi/mmole of nucleotide (i.e., about 2 x 108 dpm/mmole). Sedimentation velocity a t neu