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In vitro incorporation of deoxyribonucleoside diphosphates into DNA

✍ Scribed by James M. Pollock Jr.; Rudolf Werner

Book ID
119406087
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
334 KB
Volume
63
Category
Article
ISSN
0006-291X

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πŸ“œ SIMILAR VOLUMES

In vitro incorporation of tritium into n
In vitro incorporation of tritium into native DNA
✍ FranΓ§oise Doppler-Bernardi; Gary Felsenfeld πŸ“‚ Article πŸ“… 1969 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 540 KB

An exchange method is described for producing tritium-labeled native DNA in vitro with minimal physical damage to the DNA. Tritium-labeled calf thymus DNA prepared in this way has a specific activity of about 100 pCi/mmole of nucleotide (i.e., about 2 x 108 dpm/mmole). Sedimentation velocity a t neu