The in vitro motility of human gliomas increases with increasing grade of malignancy

✍ Scribed by Michael R. Chicoine; Daniel L. Silbergeld

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 569 KB
• Volume: 75
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19950615)75:12<2904::aid-cncr2820751218>3.0.co;2-2

✦ Synopsis

Background. Malignant gliomas are a highly invasive disease with a dismal prognosis despite aggressive therapeutic interventions.

Methods. The authors tested the hypothesis that increasing in vitro motility of human glial tumors correlates with increasing grade of malignancy. A radial dish assay was used to quantitatively assess the brain tumor cell motility of 14 low passage cell lines derived from human glial tumors of varied malignancy, and 3 cell lines derived from human glia. The egress of cells from a central region of high tumor cell density to a region of lower tumor cell density was determined at various time points. A motility coefficient (MC), the slope of distance traveled against time, was generated by simple linear regression analysis.

Results. The MC increased with the increased histologic grade of malignancy. Generation of a t-statistic was used to determine the significance of differences in motility among 5 histopathologic groups: 10 glioblastoma (World Health Organization Grade IV) cell lines (mean MC, 0.00396), 2 mixed anaplastic oligoastrocytoma (Grade 111) cell lines (mean MC, 0.00382), 1 anaplastic astrocytoma (Grade 111) cell line (mean MC, 0.00295), 1 Grade I glioma cell line (mean MC, 0.00206), and 3 human glial cell lines (mean MC, 0.00110).