The human transmembrane secretory component (poly-Ig receptor): molecular cloning, restriction fragment length polymorphism and chromosomal sublocalization

✍ Scribed by P. Krajči; K. H. Grzeschik; A. H. M. Geurts Kessel; B. Olaisen; P. Brandtzaeg

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 870 KB
• Volume: 87
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00201717

✦ Synopsis

The human transmembrane secretory component (SC) mediates glandular translocation of polymeric IgA and IgM into exocrine secretions. A 2898-bp cDNA clone, encoding the entire sequence of the human transmembrane SC, was isolated from a colonic adenocarcinoma cell line cDNA library. The deduced amino-acid sequence had a length of 764 residues and showed an overall similarity of 56% and 64% with the rabbit and rat counterpart, respectively. A restriction fragment length polymorphism (RFLP) was found with PvuII, revealing a two-alle RFLP with an autosomal codominant inheritance pattern and allele frequencies of 0.65 and 0.35. Southern blot analysis of human-rodent somatic hybrid panels, including hybrids with translocation chromosomes carrying different parts of chromosome 1, assigned the SC gene to lq31-q42, thus confirming a previously reported provisional assignment.