The reliability of the enzyme histochemical technique, for the demonstration of arylsulphatase activity, using 6-bromo-2-naphthylsulphate as a substrate, is biochemically tested by using partly purified lysosome and microsome preparations from fresh human placenta tissue. Microsomes from frozen plac
The histochemical demonstration of arylsulphatase and acid hydrolases in carcinoma of the colon and the breast
β Scribed by Johan Koudstaal
- Book ID
- 113145643
- Publisher
- Elsevier Science
- Year
- 1975
- Weight
- 872 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0014-2964
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Polyacrylamide gel-electrophoresis was performed with an extract from cultivated skin fibroblasts. Arylsulphatase activity is measured and visualised using the biochemical substrate dehydroepiandrosterone sulphate and the histochemical substrate 6-bromo-2-naphthyl sulphate respectively. The histoche
In presenting his valuable technique for the histochemical demonstration of acid phosphatase, Gomori ( '41) remarked on the variable character of the pictures sometimes obtained. This variability is not a handicap in tissues which have a high phosphatase content, but I have found that it virtually p