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Biochemical investigation to the reliability of the histochemical demonstration of microsomal arylsulphatase activity in cryostat sections

✍ Scribed by C. M. Loos; A. J. Breda; A. E. F. H. Meijer; A. C. Jöbsis

Publisher
Springer
Year
1981
Tongue
English
Weight
264 KB
Volume
73
Category
Article
ISSN
1432-119X

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✦ Synopsis

Polyacrylamide gel-electrophoresis was performed with an extract from cultivated skin fibroblasts. Arylsulphatase activity is measured and visualised using the biochemical substrate dehydroepiandrosterone sulphate and the histochemical substrate 6-bromo-2-naphthyl sulphate respectively. The histochemical substrate was hydrolysed at Rf = 0.49 and 0.58 while the biochemical substrate was hydrolysed only at 0.49. We conclude that two different microsomal arylsulphatases exist: a sulphatase able to hydrolyse steroid sulphatases (Rf = 0.49) and one unable to hydrolyse steroid sulphatases (RF = 0.58). In consequence if is recommended to carry out an electrophoresis experiment after the histochemical investigation, in order to discriminate between these two types of sulphatase.

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