The Hippo Pathway: Methods and Protocols

✍ Scribed by Alexander Hergovich

Publisher: Springer New York,Humana Press
Year: 2019
Tongue: English
Leaves: 371
Series: Methods in Molecular Biology 1893
Edition: 1st ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level. Beginning with a section on Drosophila genetics, the volume continues with parts exploring molecular and cell biological studies of the Hippo pathway, the use of structural biology and biochemistry, and a brief look at the Hippo pathway in mouse models. Written for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, The Hippo Pathway: Methods and Protocols equips newcomers and specialists alike with key methodologies to accurately define the status of Hippo signaling in their experimental settings.

✦ Table of Contents

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
The Power of Drosophila Genetics: The Discovery of the Hippo Pathway (Rewatee Gokhale, Cathie M. Pfleger)....Pages 3-26
Drosophila Genetics: The Power of Genetic Mosaic Approaches (Mardelle Atkins)....Pages 27-42
Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues (Alexander D. Fulford, Paulo S. Ribeiro)....Pages 43-51
Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs (Jiajie Xu, Ting Su, Sherzod A. Tokamov, Richard G. Fehon)....Pages 53-59
Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence (Cordelia Rauskolb, Kenneth D. Irvine)....Pages 61-73
Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions (Yurika Matsui, Zhi-Chun Lai)....Pages 75-85
Front Matter ....Pages 87-87
Immunohistochemistry to Study YAP in Human Tissue Samples (Franziska Haderk, Victor Olivas, Trever G. Bivona)....Pages 89-95
Immunofluorescence Study of Endogenous YAP in Mammalian Cells (Valentina Rausch, Carsten G. Hansen)....Pages 97-106
Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells (Nathan M. Kingston, Andrew M. Tilston-Lunel, Julia Hicks-Berthet, Xaralabos Varelas)....Pages 107-113
Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors (Maria Chatzifrangkeskou, Eric O’Neill)....Pages 115-119
Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells (Sirio Dupont)....Pages 121-135
Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells (Xiaolei Cao, Bin Zhao)....Pages 137-152
HTRF® Total and Phospho-YAP (Ser127) Cellular Assays (Diana Zindel, Claire Vol, Odile Lecha, Isabelle Bequignon, Merve Bilgic, Marion Vereecke et al.)....Pages 153-166
Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids (Yoichi Asaoka, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp Heisenberg, Makoto Furutani-Seiki)....Pages 167-181
Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview (Sirio Dupont)....Pages 183-202
CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels (Ryan J. Quinton, Neil J. Ganem)....Pages 203-214
Hippo Pathway Regulation by Tyrosine Kinases (Nina Reuven, Matan Shanzer, Yosef Shaul)....Pages 215-236
Front Matter ....Pages 237-237
Structural and Biochemical Analyses of the Core Components of the Hippo Pathway (Lisheng Ni, Xuelian Luo)....Pages 239-256
Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro (Kui Lin, Geng Wu)....Pages 257-272
GST Pull-Down Assay to Measure Complex Formations (Sun-Yong Kim, Toshio Hakoshima)....Pages 273-280
Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag (Rui Chen, Steven W. Plouffe, Kun-Liang Guan)....Pages 281-287
Quantifying the Kinase Activities of MST1/2 (Niamh A. O’Driscoll, David Matallanas)....Pages 289-304
Measuring the Kinase Activities of the LATS/NDR Protein Kinases (Alexander Hergovich)....Pages 305-317
MST1/2 Kinase Assays Using Recombinant Proteins (Marta Gomez, Yavuz Kulaberoglu, Alexander Hergovich)....Pages 319-331
Front Matter ....Pages 333-333
Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development (Tristan Frum, Amy Ralston)....Pages 335-352
The Hippo Signaling Pathway in Regenerative Medicine (Lixin Hong, Yuxi Li, Qingxu Liu, Qinghua Chen, Lanfen Chen, Dawang Zhou)....Pages 353-370
Back Matter ....Pages 371-374

✦ Subjects

Life Sciences; Biochemistry, general

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