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The High-Resolution NMR Structure of the Early Folding Intermediate of the Thermus thermophilus Ribonuclease H

โœ Scribed by Zheng Zhou; Hanqiao Feng; Rodolfo Ghirlando; Yawen Bai


Book ID
116663896
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
921 KB
Volume
384
Category
Article
ISSN
0022-2836

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Strategy for trapping intermediates in t
โœ Kunihiro Kuwajima; Peter S. Kim; Robert L. Baldwin ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 567 KB

The major unfolded form of ribonuclease A is known to show well-populated structural intermediates transiently during folding a t 0"-1O0C. We describe here how the exchange reaction between D20 and peptide NH protons can be used to trap folding intermediates. The protons protected from exchange duri