Early intermediates in the PDI-assisted folding of ribonuclease A
โ Scribed by Floriana Vinci; Margherita Ruoppolo; Piero Pucci; Robert B. Freedman; Gennaro Marino
- Book ID
- 111753772
- Publisher
- Cold Spring Harbor Laboratory Press
- Year
- 2008
- Tongue
- English
- Weight
- 376 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0961-8368
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๐ SIMILAR VOLUMES
The major unfolded form of ribonuclease A is known to show well-populated structural intermediates transiently during folding a t 0"-1O0C. We describe here how the exchange reaction between D20 and peptide NH protons can be used to trap folding intermediates. The protons protected from exchange duri
This report describes the use of fluorescence energy transfer between an intrinsic energy donor (tryptophan 177) and two chemically added acceptors to study intermediates in the folding of the beta 2 subunit of E. coli tryptophan-synthase. Two early folding steps are thus identified and characterize