The glucose-dependent transactivation activity of ABF1 on the expression of theTDH3gene in yeast

✍ Scribed by So Young Jung; Hae Yong Yoo; Young Ho Kim; Jiyoung Kim; Hyune Mo Rho

• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 671 KB
• Volume: 27
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00352099

✦ Synopsis

Autonomously replicating sequence binding factor 1 (ABF1) has been implicated in the control of a variety of gene expressions in Saccharomyces cerevisiae. In this paper evidence is presented that ABF1 is involved in the glucose-dependent expression of the TDH3 gene which encodes glyceraldehyde-3-phosphate dehydrogenase. ABF1 binds to consensus sites located between -420 and -250, and between +77 and +200, and acts as a transactivator in an orientation-independent manner on both upstream and downstream sites. TDH3-1acZ fusions having an ABF1 consensus motif showed glucose-dependent expression of TDH3, whereas in the abfl mutant strain JCA35 glucose-dependent expression disappeared, These findings suggest that ABF1 functions as a glucose-dependent transactivator for the expression of the TDH3 gene.