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The Giemsa banding patterns of the standard and four reconstructed karyotypes ofVicia faba

✍ Scribed by Peter Döbel; Rigomar Rieger; Arnd Michaelis

Publisher
Springer
Year
1973
Tongue
English
Weight
1014 KB
Volume
43
Category
Article
ISSN
0009-5915

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✦ Synopsis

The Giemsa banding patterns of the standard karyotype of Vicia/aba and of four new karyotypes with easily interdistinguishable chromosomes due to interchanges and inversions are described and compared with the data of other authors on preferential Giemsa staining in Vicia ]aba. All karyotypes contain 14 easily reproducible marker bands which characterize chromosome segments known to be heterochromatic. It is shown that the preferential Giemsa staining of chromosome regions is a valuable tool for the localization of transloeation and inversion points in the chromosomes of the reconstructed Vicia karyotypes. A close correlation exists between banding patterns, segment extension by incorporation into chromosomal DNA of azacytidine and mutagen-specific clustering of induced chromatid aberrations in the new karyotypes.

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