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The genes of both subunits of ribulose-1,5-bisphosphate carboxylase constitute an operon on the plastome of a red alga

✍ Scribed by Klaus Valentin; Klaus Zetsche

Book ID
104752578
Publisher
Springer-Verlag
Year
1989
Tongue
English
Weight
617 KB
Volume
16
Category
Article
ISSN
0172-8083

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✦ Synopsis

Plastid (pt) DNA from the red alga Porphyridium aerugineum was purified by CsC1 gradient centrifugation. An EcoRI library of the ptDNA was screened with a gene probe specific for the gene encoding the large subunit (LSU) of ribulose-l,5bisphosphate carboxylase/oxygenase (Rubisco EC 4.1.1.39) from spinach. A 5.8 kb EcoRI clone containing the LSU gene (rbcL) was isolated and the DNA sequence of the Porphyridiurn rbcL gene and its flanking regions was determined. An open reading flame was found 130bp downstream from the rbcL gene that shows homology to genes coding for the small subunit of Rubisco (rbcS) from higher plants and cyanobacteria. Both genes (rbcL + rbcS) are cotranscribed. Comparison of rbcL and rbcS sequences from Porphyridiurn, higher plants and cyanobacteria seems to reveal a remarkable evolutionary distance between the plastids of the red algae (rhodoplasts), chloroplasts and cyanobacteria.

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