Genes for the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase constitute a single operon in a cyanobacterium Anacystis nidulans 6301
✍ Scribed by Shinozaki, Kazuo ;Sugiura, Masahiro
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 782 KB
- Volume
- 200
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
The structure and transcription of the genes for the large (LS) and small subunits (SS) of ribulose-l,5-bisphosphate carboxylase/oxygenase of Anacystis nidulans 6301 were studied. The SS gene is located 93 base pairs (bp) downstream from the LS gene on the same DNA strand. Northern hybridization analysis revealed that the LS and SS genes are transcribed as a 2.2 kilobase (kb) mRNA. The 5' end of the mRNA was mapped 158 160 bp upstream from the ATG initiation codon of the LS gene and its 3' end 224-230 bp downstream from the TAA termination codon of the SS gene. These observations indicate that the LS and SS genes constitute a single operon. Upstream from the LS gene, we found two open reading frames on the same DNA strand. These are overlapped by 40 nucleotides and portions of their amino acid sequences resemble each other