The Fluorophore 4′,6-Diamidino-2-phenylindole (DAPI) Induces DNA Folding in Long Double-Stranded DNA
✍ Scribed by Dr. Maria Rosa Beccia; Dr. Tarita Biver; Alberto Pardini; Jacopo Spinelli; Prof. Fernando Secco; Prof. Marcella Venturini; Dr. Natalia Busto Vázquez; Prof. Maria Pilar Lopez Cornejo; Victoria Isabel Martin Herrera; Prof. Rafael Prado Gotor
- Book ID
- 112006632
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 644 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1861-4728
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4'-6-diamidino-phenylindole (DAPI) which is used for the detection of mycoplasmic infections of cell cultures or cytofluorometric investigations proved to be a sensitive agent for the fluorometric assay of alkali extracted DNA in cultured cells and human epidermis. Various experiments to optimize as
Nonradiolabeled cells derived from animals or cell cultures can be examined in nucleotd gradients labeled with 4',6-diamidino-2-phenylindole to quantitate extremely low levels of DNA strand breaks and DNA superhelicity. In vitro: 4',6-Diamidino-2-phenylindole detection of DNA in nucleoid gradients r