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Estimation of total DNA in crude extracts of plant leaf tissue using 4′,6-diamidino-2-phenylindole (DAPI) fluorometry

✍ Scribed by L.Slade Lee; Helen M. Garnett


Book ID
116098176
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
613 KB
Volume
26
Category
Article
ISSN
0165-022X

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4'-6-diamidino-phenylindole (DAPI) which is used for the detection of mycoplasmic infections of cell cultures or cytofluorometric investigations proved to be a sensitive agent for the fluorometric assay of alkali extracted DNA in cultured cells and human epidermis. Various experiments to optimize as