The membrane potential of cultured bovine aortic endothelial cells was assessed by a fluorescent probe as an alternative to direct methods. We used the fluorescent cationic dye rhodamine 6G, a lipophilic probe with high permeability in cell membranes. A linear relationship was obtained between fluor
The fluorescent probe method using cyanine dye to determine the membrane potential in cells
β Scribed by Tadaaki Kakutani; Ryuichi Nonaka; Yoshiyuki Murata; Ichiro Obi; Mitsugi Senda
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 789 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1567-5394
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## Abstract Under controlled conditions, fluorescent cyanine dyes can be used to mesure membrane potentials of cell suspensions. Similar changes in membrane potential can be followed both with fluorescent dyes and electrophysiological probes in response to changes in the ion composition of the medi
The membrane potential of cultured rat sciatic nerve Schwann cells was determined with conventional microelectrode and voltage-sensitive fluorescent dye, Di-S-C,(5), optical techniques. The value for membrane potential obtained with microelectrodes was -42.1 \* 4.7 mV (n = 8). Using optically determ