The fate of the donor DNA after conjugation in Escherichia coli K12: Influence of the growth rate of the recipient
โ Scribed by Bergmans, Hans E. N. ;Hoekstra, Wiel P. M.
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 519 KB
- Volume
- 158
- Category
- Article
- ISSN
- 0026-8925
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We have developed an experimental system for studying concomitantly the fate of the donor DNA and the process of recombination after conjugation in Escherichia coli. We used a set of Hfr and F-strains carrying complementing lacZ mutations. Expression of the lacZ allele on the chromosomal fragment de
We provide evidence that, in terms of transcriptional organisation, the rpoBC operon carried by lambdarifd 18 accurately represents the corresponding region of the E. coli K12 chromosome. A restriction fragment of E. coli K12 chromosomal DNA carrying the genes rpoBC (encoding the beta and beta' subu