On the mechanism of conjugation in Escherichia coli K12
β Scribed by Bresler, S. E. ;Lanzov, V. A. ;Likhachev, V. T.
- Publisher
- Springer
- Year
- 1973
- Tongue
- English
- Weight
- 351 KB
- Volume
- 120
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In accord with the observations of other workers, unselected marker analysis of Escherichia coli K-12 transconjugants isolated from matings involving several different Hfr strains as donors has shown that most genetic exchanges are clustered either near the selected marker or near the origin of the
R factors of the N incompatibility group apparently transfer at low frequencies (often 10(-6)-10(-5) per donor cell) in 30 min liquid matings. However, the level of transfer is greatly increased when donor-recipient mixtures are held on solid medium selective for the recipient only, prior to transco
Conjugational recombination in Escherichia coli was investigated by measuring lacZ+ product, beta-galactosidase, in crosses between lacZ mutants. Enzyme production in both Hfr and F-prime crosses was detected very soon after transfer of the donor lacZ allele. The level of enzyme activity was reduced
A series of deletions, Mu insertions and point mutations affecting the malK-lamB operon have been isolated. They were used to establish a deletion map of this operon, which could be divided in 27 intervals, with 16 in malK and 11 in lamB. One interesting feature of this map is the lack of randomness