The expression of recombinant proteins in yeasts
โ Scribed by Peter E Sudbery
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 716 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0958-1669
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โฆ Synopsis
The expression of recombinant proteins in yeasts Peter E Sudbery
The methylotrophic yeasts Hansenula polymorpha and Pichia pastoris are rapidly becoming the systems of choice for the expression of recombinant proteins in yeast. However, the powerful genetic techniques available in Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe are still exploited to establish models to study medically important cell processes and screen for pharmacologically active compounds.
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Maximizing efficiency for the secretion of proteins from yeast requires an understanding of the rate limiting stages in secretion that can result from high levels of gene expression. Recent progress in this area has produced a number of improvements in yeast expression systems for protein secretion.
The Pichia pastoris expression system offers economy, ease of manipulation, the ability to perform complex post-translational modifications, and high expression levels. Using this system, recent advances have been made in the quality of recombinant proteins in fermenter culture and in the quality of