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The expanding role of poly(ADP-ribose) metabolism: current challenges and new perspectives

✍ Scribed by Jean-Philippe Gagné; Michael J Hendzel; Arnaud Droit; Guy G Poirier

Book ID
113524777
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
345 KB
Volume
18
Category
Article
ISSN
0955-0674

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✍ Nasreen Aboul-Ela; Elaine L. Jacobson; Myron K. Jacobson 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 981 KB

Methods are described for the radiolabeling and determination of NAD+, poly(ADP-ribose), and protein-bound monomers of ADP-ribose in cultured mammalian cells. The adenine nucleotide pools of confluent monolayer cell cultures are radiolabeled using high-specific-activity [3H]adenine. Following any de