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The existence of triphosphorylated 5′-ends in virus-specific RNA isolated from SV-40 transformed cells

✍ Scribed by G. P. Georgiev; O. P. Samarina; V. L. Mantieva

Publisher
Springer
Year
1976
Tongue
English
Weight
471 KB
Volume
3
Category
Article
ISSN
0301-4851

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✦ Synopsis

The question about the nature of promoters in the transcriptional units containing SV-40 sequences in transformed cells was analyzed. It was found that the pulse-labeled RNA hybridizing to SV-40 DNA contains small but significant amounts of triphosphorylated 5 '-ends detected as pppGp in alkaline hydrolyzates of this RNA. In another series of experiments the fragments of RNA containing triphosphorylated 5'-ends about 100 nucleotides in length have been isolated by hydroxyapatite chromatography. Some of them form hybrids with SV-40 DNA. The conclusion is drawn that at least some of SV-40 promoters are used for transcription initiation in SV-40 transformed cells.

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